Glycoprotein Ibα (GPIbα), also known as CD42b, is a component of the GPIb-V-IX complex on platelets. The GPIb-V-IX complex binds von Willebrand factor, allowing platelet adhesion and platelet plug formation at sites of vascular injury. These anti-CD42b monoclonal antibodies are generated in hybridoma cells isolated from BALB/c GPIbα knockout mice immunized with wild-type mouse platelets.
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Characterization of anti-GPIbα monoclonal antibodies
Monoclonal antibodies were generated in GPIbα-/- mice (immunized with wild-type platelets). Subtype and binding to platelets were analyzed by flow cytometry using washed platelets pre-incubated with 5 μg/mL of monoclonal antibodies and then incubation with FITC-conjugated anti-mouse IgM, IgG, IgG1, IgG2a, IgG2b, and IgG3 secondary antibodies. M: mouse, P: pig